Plasmid_Backbone

Part:BBa_K4320009:Design

Designed by: MIT iGEM 2022   Group: iGEM22_MIT   (2022-10-11)


pYTK_098


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
    Illegal NotI site found at 3466
    Illegal NotI site found at 5229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1214
    Illegal BsaI.rc site found at 196
    Illegal SapI.rc site found at 380
    Illegal SapI.rc site found at 2090


Design Notes

N/A


Source

Duber YTK MoClo Kit

References